Hum Mol Genet. 2016 Sep 1;25(17):3836-3848. doi: 10.1093/hmg/ddw230.

The progressive ankylosis protein ANK facilitates clathrin– and adaptor-mediated membrane traffic at the trans-Golgi network-to-endosome interface.

Seifert W1, Posor Y2, Schu P3, Stenbeck G4, Mundlos S5,6,7, Klaassen S8,9, Nürnberg P10, Haucke V2, Kornak U5,6,7, Kühnisch J11,6,8.
1 Institute of Vegetative Anatomy, Charité – Universitätsmedizin Berlin, Germany.
2 Department of Molecular Pharmacology and Cell Biology, Leibniz-Institut für Molekulare Pharmakologie (FMP), Berlin, Germany.
3 Department of Cellular Biochemistry, Universitätsmedizin Georg-August University, Göttingen, Germany.
4 College of Health and Life Sciences, Brunel University, Uxbridge, United Kingdom.
5 Institute for Medical and Human Genetics, Charité – Universitätsmedizin Berlin, Germany.
6 FG Development and Disease, Max-Planck-Institute for Molecular Genetics, Berlin, Germany.
7 Berlin-Brandenburg Center for Regenerative Therapies, Charité – Universitätsmedizin Berlin, Germany.
8 Experimental and Clinical Research Center (ECRC) | Max-Delbrück-Centrum for Molecular Medicine (MDC), Charité – Universitätsmedizin Berlin, Berlin, Germany.
9 Department of Pediatric Cardiology, Charité – Universitätsmedizin Berlin, Germany and.
10 Cologne Center for Genomics (CCG), University of Cologne, Germany.
11 Institute for Medical and Human Genetics, Charité – Universitätsmedizin Berlin, Germany jirko.kuehnisch@mdc-berlin.de.

 

Abstract

Dominant or recessive mutations in the progressive ankylosis gene ANKH have been linked to familial chondrocalcinosis (CCAL2), craniometaphyseal dysplasia (CMD), mental retardation, deafness and ankylosis syndrome (MRDA). The function of the encoded membrane protein ANK in cellular compartments other than the plasma membrane is unknown. Here, we show that ANK localizes to the trans-Golgi network (TGN), clathrin-coated vesicles and the plasma membrane. ANK functionally interacts with clathrin and clathrin associated adaptor protein (AP) complexes as loss of either protein causes ANK dispersion from the TGN to cytoplasmic endosome-like puncta. Consistent with its subcellular localization, loss of ANK results in reduced formation of tubular membrane carriers from the TGN, perinuclear accumulation of early endosomes and impaired transferrin endocytosis. Our data indicate that clathrin/AP-mediated cycling of ANK between the TGN, endosomes, and the cell surface regulates membrane traffic at the TGN/endosomal interface. These findings suggest that dysfunction of Golgi-endosomal membrane traffic may contribute to ANKH-associated pathologies. © The Author 2016.

PMID: 27466194; DOI: 10.1093/hmg/ddw230

Pubmed

 

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