Placenta. 2016 Oct;46:26-30. doi: 10.1016/j.placenta.2016.08.077. Epub 2016 Aug 20.

Vasoactivity of the rat endovascular trophoblast.

Ariel I1, Skarzinski G2, Belzer V3, Abu Ahmad W4, Abassi Z5, Bursztyn M6.
1Department of Pathology, Hadassah-Hebrew University Medical Center, Mount Scopus, Israel. Electronic address: ilanaar@ekmd.huji.ac.il.
2Department of Pathology, Hadassah-Hebrew University Medical Center, Mount Scopus, Israel; Department of Medicine, Hadassah-Hebrew University Medical Center, Mount Scopus, Israel.
3Department of Surgery, Hadassah-Hebrew University Medical Center, Mount Scopus, Israel.
4Hebrew University-Hadassah School of Public Health, Ein Kerem, Jerusalem, Israel.
5Department of Physiology, The Bruce Rappaport Faculty of Medicine, and The Department of Laboratory Medicine, Rambam Health Center, Haifa, Israel.
6Department of Medicine, Hadassah-Hebrew University Medical Center, Mount Scopus, Israel.

Abstract

INTRODUCTION:

Rat endovascular trophoblasts (EVasT) express smooth muscle (SM) proteins and contract ex vivo upon exposure to endothelin-1 (ET1) via receptors A and B (ETA, ETB). Presently, we investigated the EVasT response to NOS inhibition (N-Nitro-l-arginine methyl ester hydrochloride, l-NAME), and potentiation by NO donor [S-Nitroso-N-Acetyl-D,l-Penicillamine (SNAP)] following KCl precontraction. M&M: Luminal surface area (LSA) of remodeled spiral artery rings (SAR) devoid of SM was measured ex vivo upon exposure to l-NAME alone; l-NAME and ET1 representing the combined contractile effect of both ET1 receptors; l-NAME with ET1 and ETA antagonist, representing the isolated contractile effect via ETB. In another experiment we administered SNAP to KCl precontracted SAR. Statistical analysis was performed using 2-way mixed ANOVA and repeated measures.

RESULTS:

l-NAME reduced LSA by 2.22%, 95% CI [0.83%, 3.60%] compared with control. ET1 and l-NAME reduced LSA immediately, compared with a plateau at 60min by ET1 alone. The isolated ET1 constrictive effect via ETB, reduced LSA by 5.94%; 95% CI [3.47%, 8.41%], significantly more than that obtained via ETA following 36 min of the experiment by 0.88%; 95%CI [0.09%, 1.67%]. Addition of KCl reduced LSA by 11.9%, 95% CI [9.6%, 14.1%]. Addition of SNAP increased LSA by 3.0%, 95% CI [1.7%, 4.3%].

CONCLUSIONS:

EVasT of the rat remodeled spiral artery react to ET1 and KCl similar to vascular SM: contract via both ET1 receptors and KCl and relax by ET1 via ETB and by SNAP. This phenomenon may play a role in rat models of gestational vasoactive systems dysregulation.

KEYWORDS:

Endothelin receptors; Endovascular trophoblast; Myotrophoblast; Nitric oxide; Rat placenta; Remodeled spiral artery

PMID: 27697218; DOI: 10.1016/j.placenta.2016.08.077

 

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